VĪ“1 T cells are a subpopulation of Ī³Ī“T cells found in human dermis. In contrast to murine skin-resident Ī³Ī“T cells, much less is known regarding their role and function in skin health and disease. Here we report the successful integration of VĪ“1 T cells into long-term fibroblast-derived matrix skin equivalents (SE). We isolated VĪ“1 T cells from human blood, where they are rare, and established conditions for the integration and maintenance of the freshly isolated VĪ“1 T cells in the SEs. Plated ā¦