Severe acute graft-versus-host disease (aGVHD) is associated with significant mortality and morbidity, especially in steroid-resistant (SR) cases. Spatial transcriptomic technology can elucidate tissue-based interactions in vivo and possibly identify predictors of treatment response. Tissue sections from thirty-two treatment naïve patients with biopsy-confirmed lower gastrointestinal aGVHD were obtained. The GeoMxTM digital spatial profiler was used to capture transcriptome profiles of …